RECOMBINANT HUMAN TRYPTASE Recombinant Human Tryptase (rhTryptase; Cat.# G7061) is expressed as a fully active mature enzyme in Pichia pastoris by methanol induction and is purified from the supernatant using affinity chromatography
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چکیده
Tryptase, a 135kDa tetrameric serine protease, was originally isolated from lung mast cells (MC). This enzyme is stored in and released from MC granules upon activation. Tryptase is an exquisite marker in heterogeneous populations of MC, allowing for their differentiation from basophils. Because tryptase is the principal protein mediator component of mast cell granules, accounting for 23% of the total cellular protein (1), it is abundant in unstimulated MC and surrounding degranulated MC.
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